منابع مشابه
Plasminogen assay by a haemagglutination inhibition technique.
Formaldehyde or gluteraldehyde cells treated with tannic acid were coated with plasminogen. These cells are agglutinated by plasminogen antiserum and this reaction can be quantitatively inhibited by purified plasminogen, plasma, and euglobulin. Correlation between this haemagglutination assay and a caseinolytic method is good. The sensitivity of the technique is high and compares favourably wit...
متن کاملA colorimetric assay for releasable plasminogen activator.
We describe an equilibrium assay for measuring release of plasminogen activator form blood-vessel walls and report data from 125 individuals free of overt thromboembolic disease. Excess human plasminogen is added to the euglobulin fraction of plasma obtained before and after venous occlusion at mean systolic pressure. To measure plasmin generation in these samples, we used the chromogenic plasm...
متن کاملA Simple Quantitative Assay of Vascular Plasminogen Activator
A simple method for the quantitative assay of vascular plasminogen activator is described. From 24 chronic renal failure patients who were undergoing surgery for A/V fistular or shunt construction, small pieces of artery (mean 7.8 mg, range 3.0-18.5 mg) and vein (mean 4.5 mg, range 3.0-8.8 mg) were used. The fibrinolytic activity was assayed on a well-controlled fibrin plate by only distilled w...
متن کاملThe assay of human plasminogen with casein as substrate.
Casein has been widely used for the assay of proteolytic enzymes since Kunitz (1947) described his procedure for the measurement of the activity of trypsin and chymotrypsin. Several procedures have since been designed for the assay of plasmin, the most widely used being that of Remmert & Cohen (1949). These workers activated plasminogen with excess of streptokinase in the presence of casein and...
متن کاملA simple quantitative assay of tissue plasminogen activator.
A simple method for the quantitative assay of tissue plasminogen activator is described. Human veins and uterus obtained at operation are disintegrated in a membrane disintegrator at -70 degrees C and a known weight of the powder, suspended in buffered saline and thoroughly mixed. Assay of the dilutions of this homogenate on isotope-labelled fibrin clots gives straight line plots of log weight ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1968
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/14.3.262